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Ray Wright Professor and Animal Scientist Fellow, Health Sciences Center, Spokane Ph.D., University of California Phone: (509)335-4125 E-mail: raywright@wsu.edu

EARLY EMBRYO DEVELOPMENT. Our laboratory is interested in early embryo metabolism and the utilization of energy substrates. The work involves the identification of enzymes in specific pathways by the use of a micro-volume metabolic chamber. Study of trophoblastic vesicles has proven useful in studying embryo development as a model system. Improving the success rate and simplicity of the freezing procedure for embryos and various aspects of in vitro fertilization are areas of interest. 

Specific Research Areas:

• Utilization of glucose by preimplantation embryos and identification of specific biochemical pathways.
• Formation and study of trophoblastic vesicles in domestic animals.
• Improving the short-term culture systems for domestic animals.
• Use of sucrose and other cryoprotectants to improve freezing success rate in domestic animals.
• In vitro fertilization of domestic animals.
  

Selected Publications:

1. Ellington J.E., J. Samper, A. Jones, S.A. Oliver, K. Burnett, R.W. Wright Jr. 1999. Effects of bovine serum albumin on cryopreserved stallion sperm function during media cultures and oviduct epithelial cell culture. Amer. J. Vet. Res. 60:363-367.
2. Schneider C.S., J.E. Ellington, R.W. Wright, Jr. 1999. Effects of bulls with different field fertility on in vitro embryo cleavage and development using sperm co-culture systems. Theriogenolgy 51:1085-1098.
3. Ellington J.E., J. Samper, A.E. Jones, S.A. Oliver, K. Barnett, R.W. Wright, Jr. 1999. Interactions of cryopreserved stallion sperm and oviduct epithelial cells on their products in vitro. Anim. Reprod. Sci. 56:51-65.
4. Ellington J.E., A.E. Jones, C.M. Bavirr, C.S. Schneider, R.S. Brisbois, G.A. Hiss, R.W. Wright Jr. 1998. Human sperm function in co-culture with human, macaque or bovine oviduct epithelial cell monolayers. Human Repro. 13:2797-2804.
5. O’Fallon, J.V., R.W. Wright, Jr. and R.E. Calza. 1996. Enzymatic method for measuring subnanomolar concentrations of oxygen in gaseous or aqueous solutions. Anal. Biochem. 239:193-199.
6. Staigmiller, R.B., R.A. Bellows, G.B. Anderson, G.E. Seidel, Jr., W.D. Foote, A.R. Menino, Jr. and R.W. Wright, Jr. 1992. Superovulation of cattle with equine pituitary extract and porcine FSH. Theriogenology 37:1091-1099.
7. O'Fallon, J.V. and R.W. Wright, Jr. 1991. Methyl viologen as a preferred electron acceptor in metabolic experiments. Anal. Biol. 198.
8. Thompson, J.G.E., A.C. Simpson, P.A. Pugh, R.W. Wright, Jr. and H.R. Tervit. 1991. Glucose utilization in vivo and in vitro derived sheep embryos. Reproduction, Fertility and Development 3:132-138.
9. Hernandez-Ledezma, J.J. and R.W. Wright, Jr. 1989. Freezing of mouse embryos with a combination of cryoprotectants. Theriogenology 32:735-743.